DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta)

DNA ( cytosine - 5 ) - methyltransferase 3 B - [ Isoform 1 ]

A DNMT3B (DNMT3B , DNA (cytos ine-5)methyltransferase 3B; Swiss-Prot accession number: Q9UBC3) gene deletion construct in which exons 16–19 were deleted was used to generate Dnmt3b-null ES cells and animals. The deleted region encodes the highly conserved PC (proline cysteine) and ENV (glutamic acid, asparagine, valine) motifs that are crucial for catalytic activity [1,2]. Targeted replacement ...

DNMT 1 ( DNA ( cytosine - 5 - ) - methyltransferase 1 ) )

DNA ( cytosine - 5 ) - methyltransferase 1 - [ Isoform 1 ]

The murine equivalent of the human DNMT1 (DNA [cytosine-5]-methyltransferase 1; Swiss-Prot accession number: P26358) gene was deleted in ES cells via gene targeting [1]. Dnmt1-/cells possessed dramatically decreased genomic methylation and were viable; however, the mutation caused a homozygous lethal phenotype when introduced into the germline (see Table of experimental models for DNMT1) [1]. C...

Atlas of Genetics and Cytogenetics in Oncology and Haematology DNMT 3 B ( DNA ( cytosine - 5 - ) - methyltransferase 3 beta )

Other names: ICF, ICF1, M.HsaIIIB HGNC (Hugo): DNMT3B Location: 20q11.21 Note DNA (cytosine-5)-methyltransferase 3B (DNMT3B) is one of the three known DNA methyltransferases with catalytic activity. This enzyme is responsible for the de novo methylation of DNA, particularly during embryogenesis (Okano et al., 1999). In fact, DNMT3B adds methyl groups to CpG dinucleotides of unmethylated DNA for...

Protein-facilitated base flipping in DNA by cytosine-5-methyltransferase.

DNA methylation, various DNA repair mechanisms, and possibly early events in the opening of DNA as required for transcription and replication are initiated by flipping of a DNA base out of the DNA double helix. The energetics and structural mechanism of base flipping in the presence of the DNA-processing enzyme, cytosine 5-methyltransferase from HhaI (M.HhaI), were obtained through molecular dy...